MAP3K1 anticorps (AA 1211-1310)

N° du produit ABIN7865694

L’anticorps anti-MAP3K1 Monoclonal Souris est utilisé pour la détection de MAP3K1 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IHC, IF, ICC et FACS.

Aperçu rapide pour MAP3K1 anticorps (AA 1211-1310) (ABIN7865694)

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAP3K1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: EBS-T-008

Détail du produit anti-MAP3K1 anticorps

Épitope: AA 1211-1310

Fonction: anti-MAP3K1 mouse monoclonal, EBS-T-008, purified

Purification: affinity chromatography

Immunogène: partial recombinant MAP3K1 (aa1211-1310)

Isotype: IgG2a kappa

Information d'application

Indications d'application:

ICC/IF_dilution: assay dependent

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:100 - 1:200 (0,5-1,0 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:100-1:200 (0.5-1 μg/mL)

IHC-frozen_dilution: 1:100 - 1:200 (0,5-1,0 μg/mL )

elisa_dilution: assay dependent

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur MAP3K1

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Autre désignation: MAP3K1

Sujet: MAPKs are involved in directing cellular responses to a diverse array of potentially harmful stimuli, such as mitogens, osmotic stress, heat shock, proinflammatory cytokines, but also growth factors (mammals). Possibly located exclusively in the cell nucleus, they regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. In order to become active, they require usually multiple phosphorylation events in their activation loops, including phosphorylation by MAP2 kinases (Ste-7 kinases), which in turn are phosphorylated by the MAP3 kinase family, of which many are located at the cell membrane. Thus through this pathway, stimuli can effectively be conveyed from the cell membrane to the nucleus. Inactivation of MAPKs takes place by several phosphorylases, including dedicated phophorylases. Positive control: A431, HeLa or HL-60 cells or liver tissue.

Pathways: Signalisation MAPK, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades