S100A9 anticorps

N° du produit ABIN7865729

Cet anticorps Souris Monoclonal détecte spécifiquement S100A9 dans WB, IHC, IF, FACS et ICC. Il présente une réactivité avec des échantillons de Humain, Rat, Chien, Porc, Cobaye, Cheval, Lapin, Cat, Chévre et Singe.

Aperçu rapide pour S100A9 anticorps (ABIN7865729)

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivité: Humain, Rat, Chien, Porc, Cobaye, Cheval, Lapin, Cat, Chévre, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A9 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 47-8D3

Détail du produit anti-S100A9 anticorps

Fonction: anti-MRP14/S100A9/calgranulin B mouse monoclonal, 47-8D3, purified

Purification: affinity chromatography

Immunogène: human peripheral blood monocyte components derived by affinity chromatography with Sepharose 4B coupled to rabbit monocyte antibodies

Isotype: IgG1 kappa

Information d'application

Indications d'application:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/mL PBS recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur S100A9

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Autre désignation: MRP14/S100A9/calgranulin B

Sujet: 47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin. Positive control: Monocytes, macrophages, granulocytes.

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins