TGFA anticorps

N° du produit ABIN7865750

Cet anticorps Souris Monoclonal détecte spécifiquement TGFA dans IHC, FACS et ELISA. Il présente une réactivité avec des échantillons de Humain, Lapin et Poisson zèbre (Danio rerio).

Aperçu rapide pour TGFA anticorps (ABIN7865750)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain, Lapin, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: 1E8-G6

Détail du produit anti-TGFA anticorps

Fonction: anti-TGF-alpha mouse monoclonal, 1E8-G6, purified

Purification: affinity chromatography

Immunogène: synthetic TGF-alpha 34-43 decapeptide linked carrier

Isotype: IgM kappa

Information d'application

Indications d'application:

FACS_dilution: 0.5-1,0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF-alpha

Sujet: 1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGF-alpha (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained. Positive control: Jurkat or Ramos cells. testicular carcinoma, breast cancer.

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway