TDP1 anticorps (AA 1-499)

N° du produit ABIN7869421

Cet anticorps anti-TDP1 Polyclonal Lapin (ABIN7869421) détecte spécifiquement TDP1 dans WB, ELISA, IHC, IF et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour TDP1 anticorps (AA 1-499) (ABIN7869421)

Antigène: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TDP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-TDP1 anticorps

Épitope: AA 1-499

Fonction: TDP1 Antibody / Tyrosyl-DNA phosphodiesterase 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human TDP1 recombinant protein (Position: M1-R499) was used as the immunogen for the TDP1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TDP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TDP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur TDP1

Antigène: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Autre désignation: TDP1

Sujet: TDP1 antibody detects Tyrosyl-DNA phosphodiesterase 1, a DNA repair enzyme that removes covalently trapped topoisomerase I-DNA complexes and repairs DNA single-strand breaks. The UniProt recommended name is Tyrosyl-DNA phosphodiesterase 1 (TDP1). This enzyme maintains genome stability by resolving abortive topoisomerase I intermediates generated during DNA replication and transcription.

Functionally, TDP1 antibody identifies a 608-amino-acid nuclear enzyme that hydrolyzes the phosphodiester bond linking tyrosine residues of topoisomerase I to the 3? end of DNA. TDP1 also processes other blocked DNA termini generated by oxidative damage or chemotherapeutic agents. Its catalytic mechanism involves two histidine residues acting in a transesterification reaction, forming a transient covalent intermediate. TDP1 cooperates with PARP1 and XRCC1 in the single-strand break repair pathway to restore DNA integrity.

The TDP1 gene is located on chromosome 14q32.11 and is expressed in a broad range of tissues, with highest levels in brain, thymus, and testis. Expression increases in response to DNA damage and genotoxic stress, indicating a role in stress-induced DNA repair. TDP1 activity supports genomic maintenance in neurons, where oxidative DNA damage is frequent.

Pathologically, mutations in TDP1 cause spinocerebellar ataxia with axonal neuropathy (SCAN1), a rare neurodegenerative disorder characterized by progressive motor impairment and neuronal DNA repair deficiency. In cancer, TDP1 confers resistance to topoisomerase I inhibitors such as camptothecin and topotecan by removing drug-stabilized cleavage complexes. Research using TDP1 antibody supports studies in DNA repair, cancer pharmacology, and neurodegeneration.

TDP1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect DNA repair enzymes. NSJ Bioreagents provides TDP1 antibody reagents optimized for research in genomic maintenance, DNA damage response, and therapeutic resistance mechanisms.

Structurally, Tyrosyl-DNA phosphodiesterase 1 consists of two domains forming an alpha/beta sandwich fold that positions catalytic histidine residues in the active site. The enzyme's flexible loops accommodate diverse DNA substrates, and phosphorylation or SUMOylation modulates its repair activity. This antibody facilitates investigation of TDP1's role in DNA repair, topoisomerase regulation, and neuroprotection.

UniProt: Q9NUW8