Melanoma gp100 anticorps (AA 182-5565)

N° du produit ABIN7871468

Cet anticorps Lapin Polyclonal détecte spécifiquement Melanoma gp100 dans IHC, WB, FACS, IF et ELISA. Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Melanoma gp100 anticorps (AA 182-5565) (ABIN7871468)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Détail du produit anti-Melanoma gp100 anticorps

Épitope: AA 182-5565

Fonction: PMEL Antibody / Premelanosome protein / SILV

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human SILV/PMEL recombinant protein (Position: H182-Q5565) was used as the immunogen for the PMEL antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PMEL antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PMEL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: PMEL

Sujet: PMEL antibody detects Premelanosome protein, a structural component of melanosomes essential for melanin synthesis and pigment formation. The UniProt recommended name is Premelanosome protein (PMEL). Also known as SILV, gp100, or ME20, this glycoprotein forms amyloid fibrils that serve as scaffolds for melanin polymerization within developing melanosomes.

Functionally, PMEL antibody identifies a 661-amino-acid type I transmembrane glycoprotein synthesized in melanocytes. PMEL is cleaved by proprotein convertases into M alpha and M beta fragments, which assemble into amyloid fibrils in stage II melanosomes. These fibrils provide a matrix for eumelanin deposition, enabling proper pigment granule maturation.

The PMEL gene is located on chromosome 12p13.31 and is expressed exclusively in pigment-producing cells, including melanocytes and retinal pigment epithelium. PMEL expression is regulated by the transcription factor MITF and is upregulated during melanocyte differentiation. It plays a central role in pigment biogenesis and melanosome architecture.

Pathologically, PMEL is a target antigen in melanoma immunotherapy and an established marker for melanocytic tumors. Mutations in PMEL can cause pigment dilution phenotypes in animals due to altered fibril formation. Research using PMEL antibody supports studies in pigmentation biology, melanoma diagnostics, and organelle structure.

PMEL antibody is validated for immunohistochemistry, western blotting, and immunofluorescence to detect melanosomal structural proteins. NSJ Bioreagents provides PMEL antibody reagents optimized for pigment biology, cancer research, and cell differentiation studies.

Structurally, Premelanosome protein contains an N-terminal signal peptide, a polycystic kidney disease-like (PKD) domain, and a transmembrane domain. Its cleaved fragments aggregate to form functional amyloid fibrils. This antibody supports detailed examination of PMEL's role in melanosome assembly and pigmentation control.

UniProt: P40967