FEN1 anticorps (AA 4-300)

N° du produit ABIN7874666

Cet anticorps Souris Monoclonal détecte spécifiquement FEN1 dans WB, IF, IHC (p) et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour FEN1 anticorps (AA 4-300) (ABIN7874666)

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FEN1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 6F3F2

Détail du produit anti-FEN1 anticorps

Épitope: AA 4-300

Fonction: Flap endonuclease 1 Antibody / FEN1

Purification: Antigen affinity purified

Immunogène: Recombinant human protein (amino acids Q4-E300) was used as the immunogen for the Flap endonuclease 1 antibody.

Isotype: IgG1

Information d'application

Indications d'application: Optimal dilution of the Flap endonuclease 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the Flap endonuclease 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur FEN1

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Autre désignation: Flap endonuclease 1

Sujet: Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

UniProt: P39748

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA