PASK anticorps (C-Term)

N° du produit ABIN7877453

Cet anticorps Lapin Polyclonal détecte spécifiquement PASK dans FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour PASK anticorps (C-Term) (ABIN7877453)

Antigène: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PASK est non-conjugé

Application: Flow Cytometry (FACS)

Détail du produit anti-PASK anticorps

Épitope: C-Term

Fonction: PASK Antibody / PAS kinase

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human PASK was used as the immunogen for the PASK antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PASK antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PASK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PASK

Antigène: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Autre désignation: PASK

Sujet: PASK antibody detects PAS domain-containing serine/threonine-protein kinase, an evolutionarily conserved enzyme that functions as a nutrient and energy sensor linking cellular metabolism with transcriptional control. Encoded by the PASK gene on chromosome 2q37.3, this kinase contains a C-terminal catalytic domain and N-terminal PAS (Per-Arnt-Sim) domains that sense changes in oxygen, redox potential, and small-molecule metabolites. PASK acts as a molecular integrator that coordinates energy homeostasis, glucose metabolism, and mitochondrial biogenesis in response to nutrient availability. The PAS domains enable regulation of its kinase activity through allosteric mechanisms, allowing PASK to respond dynamically to metabolic states.

PASK is broadly expressed in liver, skeletal muscle, pancreatic islets, and the brain, tissues that exhibit strong metabolic plasticity. In hepatocytes, PASK influences glycogen synthesis and gluconeogenic pathways, while in pancreatic beta cells it modulates insulin secretion. Mice lacking PASK exhibit altered glucose tolerance, reduced hepatic glycogen storage, and impaired mitochondrial function, demonstrating its role in metabolic adaptation. PASK phosphorylates transcriptional coactivators such as PGC-1alpha and CREB-regulated transcriptional coactivator 2 (CRTC2), linking nutrient signaling with gene expression that governs oxidative metabolism.

The PASK antibody is used in metabolic research to detect PASK expression and activation across tissues. Western blotting typically identifies a ~160 kilodalton band corresponding to the full-length kinase, while immunofluorescence localizes it to the cytoplasm and nucleus depending on cell type. In mammalian systems, PASK activity rises during fasting and decreases in nutrient-replete conditions, reflecting its regulatory role in energy balance. Because dysregulation of PASK signaling has been associated with diabetes, obesity, and mitochondrial dysfunction, this antibody provides an important tool for investigating metabolic signaling networks.

Recent studies also implicate PASK in circadian rhythm regulation and differentiation of muscle and stem cells. Its PAS domains act as environmental sensors, adjusting transcriptional programs to oxygen and redox changes. PASK interacts with AMPK and mTOR pathways, highlighting its integration into major metabolic signaling circuits. The PASK antibody enables researchers to explore these regulatory interactions and to characterize PASK as a potential therapeutic target in metabolic disease and aging. NSJ Bioreagents provides this antibody validated for its applications, ensuring precise detection across human and model systems.

UniProt: Q96RG2

Pathways: Regulation of Carbohydrate Metabolic Process