Recombinant Cytochrome C anticorps (full length)

N° du produit ABIN7878109

Cet anticorps anti-Cytochrome C Monoclonal Lapin (Clone CYCS-3128R) (ABIN7878109) détecte spécifiquement Cytochrome C dans WB, FACS, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Recombinant Cytochrome C anticorps (full length) (ABIN7878109)

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CYCS-3128R

Détail du produit anti-Cytochrome C anticorps

Épitope: full length

Fonction: Recombinant Cytochrome C Antibody

Purification: Protein A affinity chromatography

Immunogène: A recombinant full-length human protein was used as the immunogen for this recombinant Cytochrome C antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the recombinant Cytochrome C antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant Cytochrome C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: Cytochrome C

Sujet: In mammalian cells, the highly conserved cytochrome C protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

UniProt: P99999

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity