Recombinant RPS6KB2 anticorps (pSer371)

N° du produit ABIN7879759

Cet anticorps anti-RPS6KB2 Monoclonal Lapin (Clone 32R04) (ABIN7879759) détecte spécifiquement RPS6KB2 dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Recombinant RPS6KB2 anticorps (pSer371) (ABIN7879759)

Antigène: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp RPS6KB2 est non-conjugé

Application: Western Blotting (WB)

Clone: 32R04

Détail du produit anti-RPS6KB2 anticorps

Épitope: pSer371

Fonction: Phospho-p70 S6 Kinase beta (Ser371) Antibody

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-P70 S6 Kinase beta (S371) was used as the immunogen for the Phospho-p70 S6 Kinase beta (Ser371) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-p70 S6 Kinase beta (Ser371) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-p70 S6 Kinase beta (Ser371) antibody at -20oC.

Détails sur RPS6KB2

Antigène: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Autre désignation: p70 S6 Kinase beta

Sujet: Phospho-p70 S6 Kinase beta (Ser371) antibody detects the phosphorylated form of ribosomal protein S6 kinase beta-1 (S6K1) at serine 371. S6K1, also called p70S6K, is a serine/threonine kinase activated downstream of mTORC1 and plays a pivotal role in protein synthesis, cell growth, and metabolism. Phosphorylation at Ser371 is an essential priming event for full kinase activation, enabling subsequent phosphorylation at the hydrophobic motif and activation loop.

Research using Phospho-p70 S6 Kinase beta (Ser371) antibody highlights the importance of this modification in cell growth and proliferation. Ser371 phosphorylation enhances kinase activity, promoting phosphorylation of ribosomal protein S6 and translation initiation factors. This activity stimulates protein synthesis, supporting anabolic metabolism and cellular hypertrophy. In cancer, aberrant activation of S6K1 contributes to uncontrolled growth and resistance to apoptosis. Elevated Ser371 phosphorylation has been observed in breast, prostate, and colorectal tumors, where it correlates with mTORC1 hyperactivation.

Phosphorylation at Ser371 is also a readout of nutrient and growth factor signaling. Under nutrient-rich conditions, mTORC1 activity drives S6K1 phosphorylation, whereas nutrient deprivation or rapamycin treatment diminishes this event. Monitoring Ser371 phosphorylation provides insight into mTOR pathway regulation in metabolic disease and therapeutic interventions. In insulin resistance and type 2 diabetes, dysregulated S6K1 signaling contributes to feedback inhibition of insulin receptor substrates, linking it to impaired glucose homeostasis.

Neurobiology studies show that S6K1 activity influences synaptic plasticity, memory, and neuronal survival. Ser371 phosphorylation may integrate mTOR activity with cognitive functions, while dysregulation is implicated in neurodegenerative disorders. In cardiovascular research, hyperactivation of S6K1 contributes to pathological hypertrophy and vascular remodeling.

Antibodies against phospho-S6K1 (Ser371) are validated for western blot, immunohistochemistry, and immunofluorescence. These reagents specifically recognize the phosphorylated form, enabling distinction between active and inactive states. Clone-based antibodies provide reproducibility for studying signaling pathways, drug responses, and disease mechanisms.

UniProt: Q9UBS0

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway