Recombinant ATG10 anticorps

N° du produit ABIN7880028

L’anticorps anti-ATG10 Monoclonal Lapin est utilisé pour la détection de ATG10 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IP.

Aperçu rapide pour Recombinant ATG10 anticorps (ABIN7880028)

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp ATG10 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: ABBD-1

Détail du produit anti-ATG10 anticorps

Fonction: ATG10 Antibody / Autophagy-related protein 10

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Apg10 (Atg10) was used as the immunogen for the ATG10 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the ATG10 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the ATG10 antibody at -20oC.

Détails sur ATG10

Antigène: ATG10 (Autophagy Related 10 (ATG10))

Autre désignation: ATG10

Sujet: ATG10 antibody detects Autophagy-related protein 10, an essential E2-like enzyme that catalyzes conjugation of ATG12 to ATG5, a key step in autophagosome formation. ATG10 plays a central role in the autophagy machinery by facilitating complex assembly required for membrane elongation and vesicle maturation. The ATG10 antibody is widely used in cell biology and metabolism research to study autophagy regulation, stress adaptation, and cellular degradation pathways.

ATG10 is encoded by the ATG10 gene located on human chromosome 5q14.1. The protein is approximately 220 amino acids long and resides in the cytoplasm, where it transiently associates with autophagic membranes. Functionally, ATG10 acts as an E2-like enzyme, transferring the ATG12 conjugate to ATG5 under the direction of E1-like enzyme ATG7. This conjugation system forms the ATG12-ATG5-ATG16L1 complex that defines the site of autophagosome formation.

The ATG10 antibody detects a 25 kilodalton band by western blot and shows diffuse cytoplasmic staining under confocal microscopy. ATG10 expression increases under nutrient deprivation and oxidative stress, linking metabolic signals to autophagy induction. Inhibition or depletion of ATG10 results in defective autophagosome biogenesis, accumulation of damaged organelles, and increased susceptibility to stress-induced apoptosis.

ATG10 also participates in noncanonical autophagy and immune signaling by modulating antigen presentation and cytokine secretion. Variations in ATG10 have been linked to cancers, inflammatory disorders, and aging-related diseases. Its regulation is tightly controlled through transcriptional activation by FOXO and ATF4, and by post-translational modifications that modulate enzyme activity.

Because of its indispensable role in the autophagy conjugation system, ATG10 provides a molecular link between stress sensing and degradative homeostasis. NSJ Bioreagents provides a validated ATG10 antibody optimized for its applications, supporting research into cellular degradation, metabolism, and survival mechanisms.

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy