CDKN1C anticorps

N° du produit ABIN7880548

Cet anticorps anti-CDKN1C Monoclonal Souris (Clone SPM308) (ABIN7880548) détecte spécifiquement CDKN1C dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CDKN1C anticorps (ABIN7880548)

Antigène: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDKN1C est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM308

Détail du produit anti-CDKN1C anticorps

Fonction: p57Kip2 Antibody

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the p57Kip2 antibody.

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal dilution of the p57Kip2 antibody to be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the p57Kip2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CDKN1C

Antigène: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Autre désignation: p57Kip2

Sujet: Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

UniProt: P49918

Pathways: Cycle Cellulaire, Dopaminergic Neurogenesis