Topoisomerase II alpha anticorps

N° du produit ABIN7882504

L’anticorps anti-Topoisomerase II alpha Polyclonal Lapin est utilisé pour la détection de Topoisomerase II alpha dans des échantillons de Humain. Il a été validé pour WB, IF, IHC (p), FACS et ICC.

Aperçu rapide pour Topoisomerase II alpha anticorps (ABIN7882504)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-Topoisomerase II alpha anticorps

Fonction: TOP2A Antibody

Séquence: EDYLYGQTTT YLTYND

Purification: Affinity purified

Immunogène: Amino acids EDYLYGQTTTYLTYND were used as the immunogen for the TOP2A antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TOP2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TOP2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet: The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 Molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1 % of the nuclear extract1. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II2.

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases