Recombinant ZYX anticorps

N° du produit ABIN7882735

L’anticorps anti-ZYX Monoclonal Lapin est utilisé pour la détection de ZYX dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, IP, ICC, IF et FACS.

Aperçu rapide pour Recombinant ZYX anticorps (ABIN7882735)

Antigène: ZYX (Zyxin (ZYX))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp ZYX est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AFOG-26

Détail du produit anti-ZYX anticorps

Fonction: ZYX Antibody / Zyxin

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Zyxin was used as the immunogen for the Zyxin antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Zyxin antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Zyxin antibody at -20oC.

Détails sur ZYX

Antigène: ZYX (Zyxin (ZYX))

Autre désignation: ZYX

Sujet: The Zyxin antibody targets Zyxin, a LIM domain-containing cytoskeletal protein encoded by the ZYX gene. Zyxin functions as a scaffold that connects the actin cytoskeleton to signaling pathways controlling cell adhesion, migration, and mechanical stress responses. It localizes to focal adhesions and stress fibers, where it interacts with actin-binding and signaling proteins to regulate cytoskeletal dynamics. The Zyxin antibody enables specific detection of this mechanosensitive protein in studies of cell motility, tissue architecture, and mechanotransduction.

Zyxin is characterized by three LIM domains at its C-terminus, which mediate protein-protein interactions, and a proline-rich N-terminal region that binds to VASP and alpha-actinin. Through these domains, Zyxin coordinates actin filament assembly and cell-matrix adhesion. The Zyxin antibody supports visualization of these interactions, allowing researchers to examine how this structural adaptor transduces mechanical cues into gene expression changes. Zyxin relocates to the nucleus in response to stress, suggesting a dual cytoplasmic and nuclear role in mechanosignaling.

As a component of focal adhesions, Zyxin participates in processes such as wound healing, embryonic development, and tissue remodeling. It interacts with proteins including LPP, LIMD1, and Ena/VASP to modulate cell shape and movement. The Zyxin antibody supports functional studies of these interactions, revealing how Zyxin integrates extracellular matrix forces with actin cytoskeletal organization. Its recruitment to sites of mechanical strain makes it a critical sensor for cellular adaptation to physical stress.

Altered Zyxin expression has been associated with several diseases, including cancer, cardiovascular dysfunction, and fibrotic disorders. Overexpression can enhance migration and invasion in certain tumors, while loss impairs cytoskeletal resilience. The Zyxin antibody is widely used to investigate its role in metastasis, cell adhesion signaling, and smooth muscle plasticity. Studies have also linked Zyxin to stretch-activated signaling in cardiac and vascular cells, highlighting its contribution to mechanobiology.

The Zyxin antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing distinct cytoplasmic and focal adhesion staining. NSJ Bioreagents provides this antibody as a validated reagent with strong specificity and reproducibility for use in cell biology, developmental, and oncology research. By enabling detailed analysis of Zyxin localization and expression, the Zyxin antibody supports discovery into the molecular coordination of cytoskeletal dynamics, adhesion signaling, and mechanical stress response.

UniProt: Q15942