SMAD2 anticorps (SMAD, Mothers Against DPP Homolog 2) (pSer465)

Details for Product anti-SMAD2 Antibody No. ABIN746048
Antigène
  • JV18
  • JV18-1
  • MADH2
  • MADR2
  • hMAD-2
  • hSMAD2
  • jv18
  • Xmad2
  • madh2
  • madr2
  • XSmad2
  • hmad-2
  • hsmad2
  • jv18-1
  • smad-2
  • fj43c06
  • wu:fj43c06
  • 7120426M23Rik
  • Madh2
  • Madr2
  • Smad-2
  • mMad2
  • MAD homolog 2
  • Mothers against DPP homolog 2
  • SMAD 2
  • Smad2
  • SMAD family member 2
  • SMAD family member 2-Z
  • SMAD family member 2 S homeolog
  • SMAD2
  • SMAD2Z
  • smad2
  • smad2.S
  • Smad2
Épitope
pSer465, pSer467
64
60
36
33
21
18
15
13
12
11
11
11
8
7
6
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
491
291
262
29
20
19
18
18
13
12
10
10
7
7
7
4
1
1
1
1
1
Hôte
Lapin
434
55
18
Clonalité
Polyclonal
Conjugué
Cet anticorp SMAD2 est non-conjugé
26
12
12
10
10
10
10
10
10
7
7
7
2
2
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
391
200
110
83
53
26
26
25
22
20
19
5
5
4
3
3
1
Options
Immunogène KLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others SMAD2 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Smad2 (SMAD2 Antibody Extrait)
Sujet

Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8), the common partner, (Smad4) that functions via its interaction to the various Smad's, and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.

Synonyms: Smad2p-Ser465/467, p-Smad2Ser465/467, Smad2p-S465/467, hMAD 2, hSMAD2, JV18 1, JV18, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, mothers against DPP homolog 2, SMAD 2, SMAD, SMAD2, SMAD2_HUMAN.

ID gène 4087
Pathways Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
Indications d'application WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months
Produit citée dans: Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." dans: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, 2016 (PubMed). Méthode utilisée: Western Blotting (WB)

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