TBK1 anticorps (pSer172)

N° du produit ABIN746363

L’anticorps Lapin Polyclonal anti-TBK1 a été validé pour WB. Il convient pour détecter TBK1 dans des échantillons de Souris, Rat et Singe. Il y a 3+ publications disponibles.

Aperçu rapide pour TBK1 anticorps (pSer172) (ABIN746363)

Antigène: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reactivité: Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TBK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-TBK1 anticorps

Épitope: pSer172

Specificité: This phosphorylation site is homologous across the listed species.

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human TBK1 around the phosphorylation site of Ser172

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-TBK1 (ABIN746363)

Hu, Pan, Cheng, Zhang, Wang, Chen, Zhu, Liu, Yang, Wu, Zhao, Huang, Zhang, Mao, Ou, Yu, Zhang, Liu, Tian, Pan, Rehman, Yin, Jia: "Autophagy Promotes Duck Tembusu Virus Replication by Suppressing p62/SQSTM1-Mediated Innate Immune Responses In Vitro." dans: Vaccines, Vol. 8, Issue 1, (2020) (PubMed).

Wan, Chen, Li, Pang, Chen, Nie, Pan, Qiao, Bao: "Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction." dans: International journal of biological macromolecules, Vol. 138, pp. 198-206, (2020) (PubMed).

Takahashi, Loo, Okada, Kamachi, Watanabe, Wakita, Watanabe, Kawamoto, Miyata, Barber, Ohtani, Hara: "Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells." dans: Nature communications, Vol. 9, Issue 1, pp. 1249, (2018) (PubMed).

Détails sur TBK1

Antigène: TBK1 (TANK-Binding Kinase 1 (TBK1))

Autre désignation: TBK1

Sujet:

Synonyms: NAK, T2K, Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, TANK-binding kinase 1, TBK1

Background: Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein.

ID gène: 29110

UniProt: Q9UHD2

Pathways: Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome