Lamin A/C anticorps (AA 430-545)

N° du produit ABIN7938325

Cet anticorps Souris Monoclonal détecte spécifiquement Lamin A/C dans WB, IF, ICC, IP et ChIP. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Singe et Hamster.

Aperçu rapide pour Lamin A/C anticorps (AA 430-545) (ABIN7938325)

Antigène: Lamin A/C (LMNA)

Reactivité: Humain, Souris, Rat, Singe, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Lamin A/C est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 3A6-4C11

Détail du produit anti-Lamin A/C anticorps

Épitope: AA 430-545

Fonction: Lamin A/C antibody (mAb) (Clone 3A6-4C11)

Purification: Culture Supernatant

Immunogène: This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A.

Isotype: IgG2a

Information d'application

Indications d'application: WB: 1:500 - 1:4000 dilution ICC/IF: 1:500 - 1:2000 dilution Both lamin A and lamin C are recognized by this sera, with lamin A (the slower migrating band on Western blot) sometimes appearing as a doublet. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. For immunofluorescence experiments we recommend using paraformaldehyde (PFA) fixation. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91189.

Restrictions: For Research Use only

Stockage

Buffer: Cell culture supernatant containing mouse IgG, 30 % glycerol, 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Lamin A/C

Antigène: Lamin A/C (LMNA)

Autre désignation: Lamin A/C

Sujet:

Background_Plain: Nuclear lamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins A and Lamins C are splice variants of the Lamin A gene. Lamin A/C (CDCD1, LMN1, EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing.

Aliases: LMNA, FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, FPLD2, LMNL1, CMT2B1, LGMD1B

Poids moléculaire: 75 kDa

NCBI Accession: NP_733821

Pathways: Apoptose, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus