Caspase 3 anticorps (C-Term) (HRP)

N° du produit ABIN7975311

Cet anticorps Lapin Polyclonal détecte spécifiquement Caspase 3 dans WB, IHC et ELISA. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Caspase 3 anticorps (C-Term) (HRP) (ABIN7975311)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 3 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-Caspase 3 anticorps (HRP)

Épitope: C-Term

Fonction: Anti-Caspase 3/CASP3 Antibody HRP Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: identical to the related mouse sequence,and different from the related rat sequence by one amino acid.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminal of human Caspase 3, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: CASP3

Sujet:

Background: Caspase 3 (caspase 3, apoptosis-related cysteine peptidase) is a caspase protein that interacts with caspase 8 and caspase 9, also known as Caspase-3, PARP CLEAVAGE PROTEASE, APOPAIN, CPP32, CPP32B, YAMA. It is a member of the cysteine-aspartic acid protease (caspase) family. PCR analysis of 16 human tissues revealed expression of full-length CASP3, as well as CASP3s at somewhat lower levels, in all tissues tested. Western blot analysis of 3 cell lines revealed the prominent CASP3 band at 32 kD and CASP3s at 20 kD. Several human cancer cell lines showed coexpression of both variants at the mRNA and protein levels. Overexpression of the catalytically inactive CASP3s by human kidney cells offered some resistance to inducers of apoptosis, and CASP3s accumulation could be enhanced with addition of proteasome inhibitors. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Alternative splicing of this gene results in two transcript variants that encode the same protein. Encoded by the CASP3 gene, CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Nicholson et al. developed a potent peptide aldehyde inhibitor and showed that it prevented apoptotic events in vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death.

Gene Full Name: caspase 3

Sequence Similarities: Belongs to the peptidase C14A family.

ID gène: 836

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation