Dihydrofolate Reductase anticorps (AA 2-187)

N° du produit ABIN7985267

Cet anticorps anti-Dihydrofolate Reductase Monoclonal Souris (Clone 3C8) (ABIN7985267) détecte spécifiquement Dihydrofolate Reductase dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour Dihydrofolate Reductase anticorps (AA 2-187) (ABIN7985267)

Antigène: Dihydrofolate Reductase (DHFR)

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dihydrofolate Reductase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: Carrier-free

Clone: 3C8

Détail du produit anti-Dihydrofolate Reductase anticorps

Épitope: AA 2-187

Fonction: Anti-DHFR Antibody (monoclonal, 3C8)

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.

Attributs du produit: Anti-DHFR Antibody.(monoclonal, 3C8)-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur Dihydrofolate Reductase

Antigène: Dihydrofolate Reductase (DHFR)

Autre désignation: DHFR

Sujet:

Background: SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: dihydrofolate reductase

Poids moléculaire: 22 kDa

ID gène: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases