IGFBP5 anticorps (AA 60-272) (HRP)

N° du produit ABIN8000226

L’anticorps anti-IGFBP5 Polyclonal Lapin est utilisé pour la détection de IGFBP5 dans des échantillons de Humain. Il a été validé pour WB, IHC et ELISA.

Aperçu rapide pour IGFBP5 anticorps (AA 60-272) (HRP) (ABIN8000226)

Antigène: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IGFBP5 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-IGFBP5 anticorps (HRP)

Épitope: AA 60-272

Fonction: Anti-IGFBP5 Antibody HRP Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: coli-derived human IGFBP5 recombinant protein (Position: A60-E272). Human IGFBP5 shares 96.7%,96.2% amino acid (aa) sequence identity with mouse,rat IGFBP5,respectively.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human IGFBP5 recombinant protein (Position: A60-E272). Human IGFBP5 shares 96.7% and 96.2% amino acid (aa) sequence identity with mouse and rat IGFBP5, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur IGFBP5

Antigène: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Autre désignation: IGFBP5

Sujet:

Background: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection is inhibitory to growth in 2 human breast cancer cell lines. IGFBP5 expression leads to G2/M cell cycle arrest and apoptosis. Stable expression of IGFBP5 in the breast cancer cell lines also inhibits the formation and growth of tumors following injection in athymic mice. It is concluded that IGFBP5 is a growth inhibitor and proapoptotic agent in breast cancer cells. Additionally, IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. It has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment (1, 2).

Gene Full Name: insulin like growth factor binding protein 5

ID gène: 3488

UniProt: P24593

Pathways: Signalisation WNT, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding