CrkL anticorps (C-Term)

N° du produit ABIN8053015

Cet anticorps anti-CrkL Polyclonal Lapin (ABIN8053015) détecte spécifiquement CrkL dans WB et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CrkL anticorps (C-Term) (ABIN8053015)

Antigène: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CrkL est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Classe de qualité: Carrier-free

Détail du produit anti-CrkL anticorps

Épitope: C-Term

Fonction: Anti-Crk-like protein CrkL Antibody

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: different from the related mouse,rat sequences by one amino acid.

Attributs du produit: Anti-Crk-like protein CrkL Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur CrkL

Antigène: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Autre désignation: CRKL

Sujet:

Background: CRKL (v-crk sarcoma virus CT10 oncogene homolog (avian)-like) also called ONCOGENE CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. While CRKL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization, the CRKL gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al. (1996) showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CRKL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL is a tyrosine kinase and an oncogene. Crkl was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.

Gene Full Name: CRK like proto-oncogene, adaptor protein

Sequence Similarities: Belongs to the CRK family.

Poids moléculaire: 39 kDa

ID gène: 1399

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor