Recombinant Choline Acetyltransferase anticorps (C-Term)

N° du produit ABIN8093089

Cet anticorps Souris Monoclonal détecte spécifiquement Choline Acetyltransferase dans IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Choline Acetyltransferase anticorps (C-Term) (ABIN8093089)

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Choline Acetyltransferase est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: R38B12

Détail du produit anti-Choline Acetyltransferase anticorps

Épitope: C-Term

Fonction: CHAT Antibody / Choline O-acetyltransferase

Immunogène: Prokaryotic recombinant protein corresponding to the C-terminal region of the human ChAT molecule was used as the immunogen for the CHAT/Choline O-acetyltransferase antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: 1. Optimal dilution of the CHAT/Choline O-acetyltransferase antibody should be determined by the researcher.

2. This CHAT/Choline O-acetyltransferase antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: CHAT/Choline O-acetyltransferase antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Détails sur Choline Acetyltransferase

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Autre désignation: CHAT

Sujet: CHAT antibody targets Choline O-acetyltransferase, a cytoplasmic enzyme encoded by the CHAT gene that catalyzes the biosynthesis of the neurotransmitter acetylcholine from choline and acetyl-CoA. Choline O-acetyltransferase is the defining molecular marker of cholinergic neurons and plays a central role in cholinergic neurotransmission throughout the central and peripheral nervous systems. Expression of CHAT is tightly associated with neuronal populations involved in motor control, autonomic regulation, and cognitive processing, including basal forebrain neurons, brainstem nuclei, spinal motor neurons, and autonomic ganglia. As a result, CHAT antibody is widely used in research focused on identifying cholinergic cell populations and mapping acetylcholine-related signaling pathways.

Choline O-acetyltransferase is localized primarily in the cytoplasm, where it associates with synaptic vesicle transport and acetylcholine synthesis machinery. The enzyme belongs to the ChAT family and exhibits conserved structural domains required for acetyl-CoA binding and catalytic activity. In addition to classical neuronal expression, Choline O-acetyltransferase has been reported in non-neuronal cell types, including epithelial cells, immune cells, and certain endocrine tissues, supporting the concept of non-neuronal acetylcholine production. These findings have expanded interest in CHAT expression beyond the nervous system, particularly in studies of cell communication, epithelial biology, and immune modulation. Clone r38B12 is designed to recognize Choline O-acetyltransferase for research use in studies examining cholinergic identity and acetylcholine biosynthesis.

Altered expression or activity of Choline O-acetyltransferase has been implicated in a range of neurological and neuromuscular disorders. Reduced CHAT expression is a hallmark feature observed in neurodegenerative diseases such as Alzheimer disease and amyotrophic lateral sclerosis, while changes in cholinergic signaling have also been linked to Parkinson disease and cognitive impairment. In developmental contexts, CHAT expression is critical for proper neuromuscular junction formation and motor neuron maturation. Beyond neurological disease, dysregulated cholinergic signaling has been explored in inflammation, cancer biology, and tissue homeostasis, reflecting the diverse roles of acetylcholine as a signaling molecule. Clone r38B12 provides a research tool for investigating Choline O-acetyltransferase expression and distribution in experimental systems relevant to neuronal function, development, and disease-associated cholinergic pathways.

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development