Recombinant MCM5 anticorps (AA 500-734)

N° du produit ABIN8093323

Cet anticorps Souris Monoclonal détecte spécifiquement MCM5 dans WB et IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant MCM5 anticorps (AA 500-734) (ABIN8093323)

Antigène: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: Carrier-free

Clone: RMCM5-13646

Détail du produit anti-MCM5 anticorps

Épitope: AA 500-734

Fonction: MCM5 Antibody / DNA replication licensing factor MCM5 (azide and preservative free)

Purification: Protein A affinity

Immunogène: A recombinant fragment (around amino acids 500-734) of human MCM5 protein (exact sequence is proprietary) was used as the immunogen for the recombinant Minichromosome maintenance protein 5/MCM5 antibody.

Isotype: IgG2b, kappa

Information d'application

Indications d'application: 1. Optimal dilution of the recombinant Minichromosome maintenance protein 5/MCM5 antibody should be determined by the researcher.

2. This recombinant Minichromosome maintenance protein 5/MCM5 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Minichromosome maintenance protein 5/MCM5 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Détails sur MCM5

Antigène: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Autre désignation: MCM5

Sujet: Minichromosome maintenance protein 5 Antibody recognizes MCM5, a nuclear DNA replication factor that functions as a core component of the MCM2-7 helicase complex. Minichromosome maintenance protein 5 antibody, also commonly referred to as MCM5 antibody in replication biology literature, targets a protein essential for origin licensing and replication fork progression. The MCM5 gene encodes one of six conserved subunits that assemble into the heterohexameric replicative helicase required for unwinding double-stranded DNA during S phase.

MCM5 plays a critical role during early G1 phase, when the pre-replication complex is established at replication origins through coordinated recruitment of ORC, CDC6, CDT1, and the MCM2-7 complex. Once activated by cyclin-dependent kinases and DDK, the helicase becomes competent for DNA unwinding, enabling replication fork formation. Because Minichromosome maintenance protein 5 remains associated with chromatin in cells that are replication-licensed, MCM5 antibody identifies cells with proliferative capacity rather than simply cells actively synthesizing DNA.

In normal tissues, MCM5 expression is confined to proliferative compartments such as basal epithelial layers, crypt bases in gastrointestinal mucosa, germinal centers of lymphoid tissue, and stem or progenitor cell populations. Differentiated cells that have exited the cell cycle show minimal nuclear staining. In neoplastic tissues, expanded nuclear expression of MCM5 correlates with increased proliferative fraction and dysregulated cell cycle control. Elevated Minichromosome maintenance protein 5 expression has been documented in colorectal carcinoma, breast carcinoma, lung carcinoma, prostate adenocarcinoma, and various hematologic malignancies, supporting its utility as a proliferation-associated marker in tumor biology research.

Structurally, MCM5 contains conserved ATPase domains characteristic of the AAA+ helicase family and participates directly in ATP-dependent DNA strand separation. Tight regulation of MCM5 activity is essential for preventing re-replication and maintaining genomic integrity. Dysregulation of replication licensing factors such as MCM5 contributes to replication stress and chromosomal instability, hallmarks of oncogenic transformation. Minichromosome maintenance protein 5 Antibody (clone rMCM5/13646) is designed to detect endogenous nuclear MCM5 in research applications, supporting investigation of DNA replication control, cell cycle progression, replication licensing dynamics, and proliferative signaling pathways in both normal and malignant tissues.

UniProt: P33992

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA