CD3 anticorps

N° du produit ABIN775704

Cet anticorps anti-CD3 est un anticorps Souris Monoclonal détectant CD3 dans FACS, IP, IHC (fro), ICC et MS. Adapté pour Humain et Non-Human Primate. Ce Primary Antibody a été cité dans 6+ publications.

Aperçu rapide pour CD3 anticorps (ABIN775704)

Highlights:

• High quality CD3 Anticorps primaire for the detection of CD3.
• Reliable product with high standard validation data

Antigène: CD3

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Mass Spectrometry (MS)

Clone: UCHT1

Détail du produit anti-CD3 anticorps

Fonction: Anti-Hu CD3 Purified

Specificité: The antibody UCHT1 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: human thymocytes followed by Sezary T cells

Isotype: IgG1 kappa

Informations sur le produit:

What can the CD3 Antibody ABIN775704 be used for?: This monoclonal CD3 Antibody detects CD3. The CD3 Antibody has been validated for various applications and can be used for the detection of CD3 and derivatives by Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Frozen Sections), Immunocytochemistry, Mass Spectrometry.

What validation data is available for this CD3 Antibody?: The Anticorps primaire is referenced in 9 publications and is characterized by a proven, very high reliability. It has currently 5 product images that show its performance in a variety of applications. The product is currently available in 100 μg quantity. CD3 Antibody for the detection of CD3 and derivatives.

What is the function of CD3?: The CD3 complex mediates signal transduction.

Information d'application

Indications d'application: Immunocytochemistry and immunohistochemistry (frozen sections): The epitope for UCHT1 is resistant to fixation.
Flow cytometry: Extracellular and intracellular staining, recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD3 (ABIN775704)

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." dans: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, (2006) (PubMed).

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." dans: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, (2005) (PubMed).

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, (2004) (PubMed).

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, (2003) (PubMed).

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, (1998) (PubMed).

Garson, Beverley, Coakham, Harper: "Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species." dans: Nature, Vol. 298, Issue 5872, pp. 375-7, (1982) (PubMed).

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway