CD28 anticorps

N° du produit ABIN775705

Cet anticorps Souris Monoclonal détecte spécifiquement CD28 dans FACS, WB, IP, IHC (fro) et ICC. Il présente une réactivité envers Humain et Non-Human Primate et a été mentionné dans 5+ publications.

Aperçu rapide pour CD28 anticorps (ABIN775705)

Antigène: CD28

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD28 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: CD28-2

Détail du produit anti-CD28 anticorps

Fonction: Anti-Hu CD28 Purified

Specificité: The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-12 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD28 (ABIN775705)

Jeong, Qiao, Nascimbeni, Hu, Rehermann, Murthy, Liang: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." dans: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, (2004) (PubMed).

Scharschmidt, Wegener, Heissmeyer, Rao, Krappmann: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." dans: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, (2004) (PubMed).

Marti, Krause, Post, Lyddane, Dupont, Sadelain, King: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, (2001) (PubMed).

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, (1999) (PubMed).

Tazi, Moreau, Bergeron, Dominique, Hance, Soler: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, (1999) (PubMed).

Détails sur CD28

Antigène: CD28

Autre désignation: CD28

Sujet: CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,TP44

ID gène: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response