CD28 anticorps

N° du produit ABIN611634

Détail du produit anti-CD28 anticorps

Antigène: CD28

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD28 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificité: The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Réactivité croisée (Details): Rat

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Mouse A20J B lymphoma cells transfected with rat CD28

Clone: JJ319

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 2-5 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD28 (ABIN611634)

Kerstan, Armbruster, Leverkus, Hünig: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, (2006) (PubMed).

van den Brandt, Wang, Reichardt: "Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling." dans: Molecular endocrinology (Baltimore, Md.), Vol. 18, Issue 3, pp. 687-95, (2004) (PubMed).

Lühder, Huang, Dennehy, Guntermann, Müller, Winkler, Kerkau, Ikemizu, Davis, Hanke, Hünig: "Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists." dans: The Journal of experimental medicine, Vol. 197, Issue 8, pp. 955-66, (2003) (PubMed).

Laskowski, Pratschke, Wilhelm, Dong, Beato, Taal, Gasser, Hancock, Sayegh, Tilney: "Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats." dans: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 2, pp. 519-27, (2002) (PubMed).

Haspot, Villemain, Laflamme, Coulon, Olive, Tiollier, Soulillou, Vanhove: "Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses." dans: Blood, Vol. 99, Issue 6, pp. 2228-34, (2002) (PubMed).

Robinson, Clandinin, Field: "R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation." dans: The Journal of nutrition, Vol. 131, Issue 7, pp. 2021-7, (2001) (PubMed).

Tacke, Hanke, Hanke, Hünig: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." dans: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).

Détails sur CD28

Antigène: CD28

Autre désignation: CD28 (CD28 Produits)

Synonymes: anticorps Tp44, anticorps CD28RNA, anticorps cd28, anticorps CD28 molecule, anticorps CD28 antigen, anticorps Cd28 molecule, anticorps CD28 molecule L homeolog, anticorps CD28, anticorps Cd28, anticorps cd28.L

Sujet: CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,Tp44

ID gène: 25660

UniProt: P31042

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response