IDH1 anticorps (Internal Region)

N° du produit ABIN783970

Cet anticorps Chèvre Polyclonal détecte spécifiquement IDH1 dans WB et EIA. Il présente une réactivité avec des échantillons de Saccharomyces cerevisiae.

Aperçu rapide pour IDH1 anticorps (Internal Region) (ABIN783970)

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivité: Saccharomyces cerevisiae

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IDH1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IDH1 anticorps

Épitope: Internal Region

Séquence: C-EPGSRHVGLD IKGQN

Specificité: Recognizes IDH1 (Yeast).

Réactivité croisée (Details): Species reactivity (expected):Saccharomyces cerevisiae S288cSpecies reactivity (tested):S.cerevisiae

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the Internal region of the protein sequence according to NP_014361.1. Genename: IDH1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur IDH1

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Autre désignation: IDH1

Sujet: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH1 contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.Synonyms: Isocitrate dehydrogenase [NAD] subunit 1, Isocitric dehydrogenase, NAD(+)-specific ICDH, YNL037C, mitochondrial

ID gène: 216

NCBI Accession: NP_014361

Pathways: L'effet Warburg