TARDBP anticorps (C-Term, Cleavage Site)

N° du produit ABIN784609

Cet anticorps Lapin Polyclonal détecte spécifiquement TARDBP dans WB et IHC (fro). Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour TARDBP anticorps (C-Term, Cleavage Site) (ABIN784609)

Antigène: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TARDBP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-TARDBP anticorps

Épitope: C-Term, Cleavage Site

Specificité: Specific for the ~ 43 kDa TDP-43 protein in Western blots of Rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in Human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated Human TDP-43.

Réactivité croisée (Details): Species reactivity (tested):Human and Rat.

Purification: Affinity Chromatography.

Immunogène: Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of Human TDP-43.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 30 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -80 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -80 °C.

Détails sur TARDBP

Antigène: TARDBP (TAR DNA Binding Protein (TARDBP))

Autre désignation: TARDBP

Sujet: TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43 kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms.Synonyms: TAR DNA-binding protein 43, TDP-43, TDP43

ID gène: 23435

NCBI Accession: NP_031401

Pathways: Positive Regulation of Peptide Hormone Secretion