IFNAR2 anticorps (Carboxyfluorescein (CFS))

N° du produit ABIN796985

L’anticorps Chèvre Polyclonal anti-IFNAR2 a été validé pour FACS. Il convient pour détecter IFNAR2 dans des échantillons de Souris.

Aperçu rapide pour IFNAR2 anticorps (Carboxyfluorescein (CFS)) (ABIN796985)

Antigène: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IFNAR2 est conjugé à/à la Carboxyfluorescein (CFS)

Application: Flow Cytometry (FACS)

Détail du produit anti-IFNAR2 anticorps (Carboxyfluorescein (CFS))

Specificité: Mouse IFNA/B R2.

Purification: Immunoaffinity purified

Immunogène: Mouse myeloma cell line NS0- derived recombinant human IFN-a/b R2 extracellular domain

Isotype: IgG

Information d'application

Indications d'application: Approved: Flo

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Mouse

Procédure de l'essai:

Flow Cytometry Validation
This antibody has been tested for flow cytometry using A20 cells.

1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at room temperature. Do not wash excess blocking IgG from this reaction.
   
2. After blocking, 10ul of conjugated antibody was added to 1 - 2.5 x 10e5 cells and incubated for 30 minutes at room temperature.
   
3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
   
4. The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
   
5. Cell surface expression of IFN-a/b R2 was determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515 - 545 nm.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, up to 0.5 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles and prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 4°C for up to 1 year.

Date de péremption: 12 months

Détails sur IFNAR2

Antigène: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Autre désignation: IFNAR2

Sujet: Name/Gene ID: IFNAR2
Family: Interferon Receptor

Synonyms: IFNAR2, IFN-alpha/beta receptor 2, IFN-alpha-REC, IFN-alpha binding protein, IFN-R, IFN-R-2, IFNARB, Type I interferon receptor 2, IFNABR

ID gène: 3455

UniProt: P48551

Pathways: Signalistation JAK/STAT, Hepatitis C