Leptin anticorps

N° du produit ABIN799626

Cet anticorps anti-Leptin Polyclonal Lapin (ABIN799626) détecte spécifiquement Leptin dans WB et ELISA. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Leptin anticorps (ABIN799626)

Antigène: Leptin (LEP)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Leptin est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Leptin anticorps

Specificité: Recombinant mouse leptin 16000 MW produced in E. coli.

Purification: Protein G purified

Immunogène: Recombinant mouse LEP / Leptin.

Type of Immunogen: Recombinant protein

Information d'application

Indications d'application: Approved: ELISA (1:2400), WB (1:200 - 1:400)

Usage: This IgG fraction of anti-Mouse Leptin has been tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes. This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2400 is suggested for this product.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Détails sur Leptin

Antigène: Leptin (LEP)

Autre désignation: Leptin

Sujet: Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB

ID gène: 3952

UniProt: P41159

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process