Rhodopsin anticorps

N° du produit ABIN863085

L’anticorps Souris Monoclonal anti-Rhodopsin a été validé pour WB, IHC, ELISA, IP, ICC et IF. Il convient pour détecter Rhodopsin dans des échantillons de Boeuf (Vache). Il y a 2+ publications disponibles.

Aperçu rapide pour Rhodopsin anticorps (ABIN863085)

Antigène: Rhodopsin (RHO)

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Rhodopsin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1D4

Détail du produit anti-Rhodopsin anticorps

Specificité: Detects ~40 kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).

Réactivité croisée: Vertebrate

Purification: Protein G Purified

Immunogène: Bovine Rhodopsin

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN863084 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-Rhodopsin (ABIN863085)

Makowka, Bruegmann, Dusend, Malan, Beiert, Hesse, Fleischmann, Sasse: "Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision." dans: Nature communications, Vol. 10, Issue 1, pp. 1281, (2019) (PubMed).

Cheng, Djajadi, Molday: "Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy." dans: Methods in molecular biology (Clifton, N.J.), Vol. 935, pp. 185-99, (2012) (PubMed).

Détails sur Rhodopsin

Antigène: Rhodopsin (RHO)

Autre désignation: Rhodopsin

Classe de substances: Chemical

Sujet: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

ID gène: 509933

NCBI Accession: NP_001014890

UniProt: P02699

Pathways: Signalisation WNT, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction