CD14 anticorps (FITC)

N° du produit ABIN93966

Cet anticorps anti-CD14 est un anticorps Souris Monoclonal détectant CD14 dans FACS. Adapté pour Humain et Non-Human Primate. Ce Primary Antibody a été cité dans 10+ publications.

Aperçu rapide pour CD14 anticorps (FITC) (ABIN93966)

Antigène: CD14

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: MEM-18

Détail du produit anti-CD14 anticorps (FITC)

Fonction: Anti-Hu CD14 FITC

Specificité: The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

Réactivité croisée (Details): Non-Human Primates, Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD14 (FITC) (ABIN93966)

Goyoneche Linares, Diaz-Obregón, Granda Alacote, Castro Núñez, Castañeda Torrico, Murillo Carrasco, Liendo Liendo, Rufasto Goche, Arrunátegui Correa, de León Delgado: "Leukocyte Dysregulation and Biochemical Alterations in End-Stage Kidney Disease Patients Under Hemodialysis." dans: Diseases (Basel, Switzerland), Vol. 13, Issue 4, (2025) (PubMed).

Weiss, Lichtenauer, Kirchner, Stock, Aurich, Christ, Brockhoff, Kunz-Schughart, Jauch, Schlitt, Thasler: "Hepatic progenitor cells from adult human livers for cell transplantation." dans: Gut, Vol. 57, Issue 8, pp. 1129-38, (2008) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." dans: International immunology, Vol. 19, Issue 11, pp. 1271-9, (2007) (PubMed).

Iwaki, Nishitani, Mitsuzawa, Hyakushima, Sano, Kuroki: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." dans: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, (2005) (PubMed).

Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." dans: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, (2002) (PubMed).

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, (2001) (PubMed).

Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." dans: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, (2001) (PubMed).

Bazil, Baudys, Hilgert, Stefanová, Low, Zbrozek, Horejsí: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." dans: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, (1989) (PubMed).

Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." dans: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR

ID gène: 929

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades