CD14 anticorps

N° du produit ABIN93977

L’anticorps Souris Monoclonal anti-CD14 a été validé pour FACS, ELISA et IHC (p). Il convient pour détecter CD14 dans des échantillons de Humain. Il y a 4+ publications disponibles.

Aperçu rapide pour CD14 anticorps (ABIN93977)

Antigène: CD14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: B-A8

Détail du produit anti-CD14 anticorps

Fonction: Anti-Hu CD14 Purified

Specificité: The mouse monoclonal antibody B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on the surface of monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages.

Réactivité croisée (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Human monocytes

Isotype: IgG1

Information d'application

Indications d'application: ELISA: The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100). Flow cytometry: recommended dilution: 1 μg/mL, Immunohistochemistry (paraffin sections): recommended dilution: 2-10 μg/mL, positive tissue: PML brain sections, positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD14 (ABIN93977)

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." dans: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).

Kuusniemi, Lapatto, Holmberg, Karikoski, Rapola, Jalanko: "Kidneys with heavy proteinuria show fibrosis, inflammation, and oxidative stress, but no tubular phenotypic change." dans: Kidney international, Vol. 68, Issue 1, pp. 121-32, (2005) (PubMed).

Matasić, Dietz, Vuk-Pavlović: "Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells." dans: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 909-14, (2000) (PubMed).

Goyert, Ferrero, Rettig, Yenamandra, Obata, Le Beau: "The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors." dans: Science (New York, N.Y.), Vol. 239, Issue 4839, pp. 497-500, (1988) (PubMed).

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR

ID gène: 929

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades