CD47 anticorps

N° du produit ABIN94154

L’anticorps Souris Monoclonal anti-CD47 a été validé pour FACS, WB et IHC (fro). Il convient pour détecter CD47 dans des échantillons de Humain, Porc et Non-Human Primate. Il y a 1 publication disponible.

Aperçu rapide pour CD47 anticorps (ABIN94154)

Antigène: CD47

Reactivité: Humain, Porc, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-122

Détail du produit anti-CD47 anticorps

Fonction: Anti-Hu CD47 Purified

Specificité: The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.

Réactivité croisée (Details): Non-Human Primates, Human, Porcine

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Pureté: > 95 % (by SDS-PAGE)

Immunogène: COS-7 (African green monkey) cells

Isotype: IgM

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD47 (ABIN94154)

Smith, Patel, Seatter, Deehan, Brown, Brooke, Goodridge, Howard, Rigley, Harnett, Harnett: "A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes." dans: Blood, Vol. 102, Issue 7, pp. 2532-40, (2003) (PubMed).

Détails sur CD47

Antigène: CD47

Autre désignation: CD47

Sujet: CD47 Molecule,CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.,IAP, OA3, MER6

ID gène: 961

UniProt: Q08722