CD59 anticorps

N° du produit ABIN94199

L’anticorps Souris Monoclonal anti-CD59 a été validé pour FACS, IP et IHC (p). Il convient pour détecter CD59 dans des échantillons de Humain. Il y a 4+ publications disponibles.

Aperçu rapide pour CD59 anticorps (ABIN94199)

Antigène: CD59

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD59 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-43

Détail du produit anti-CD59 anticorps

Fonction: Anti-Hu CD59 Purified

Specificité: The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on the surface of all hematopoietic cells, it is widely present on cells in all tissues. This antibody does not compete with MEM-43/5, and has blocking activity.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Thymocytes and T lymphocytes

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 0.5-4 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: placenta.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD59 (ABIN94199)

Omidvar, Wang, Brennan, Longhi, Smith, Morgan: "Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2915-23, (2006) (PubMed).

Stefanová, Horejsí, Ansotegui, Knapp, Stockinger: "GPI-anchored cell-surface molecules complexed to protein tyrosine kinases." dans: Science (New York, N.Y.), Vol. 254, Issue 5034, pp. 1016-9, (1991) (PubMed).

Forsberg, Bazil, Stefanová, Schröder: "Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11." dans: Immunogenetics, Vol. 30, Issue 3, pp. 188-93, (1989) (PubMed).

Stefanová, Hilgert, Kristofová, Brown, Low, Horejsí: "Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol." dans: Molecular immunology, Vol. 26, Issue 2, pp. 153-61, (1989) (PubMed).

Détails sur CD59

Antigène: CD59

Autre désignation: CD59

Sujet: CD59 Molecule,CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.,MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

ID gène: 966

UniProt: P13987

Pathways: Système du Complément