CD59 anticorps

N° du produit ABIN94203

Cet anticorps anti-CD59 est un anticorps Souris Monoclonal détectant CD59 dans WB, FACS, IP et IHC (p). Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour CD59 anticorps (ABIN94203)

Antigène: CD59

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD59 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-43-5

Détail du produit anti-CD59 anticorps

Fonction: Anti-Hu CD59 Purified

Specificité: The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells, it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

Réactivité croisée (Details): Mouse, Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Thymocytes and T lymphocytes

Isotype: IgG2b

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 0.5-4 μg/mL, positive control: blood.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: mouse spleen, non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD59 (ABIN94203)

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." dans: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

Bodian, Davis, Morgan, Rushmere: "Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59." dans: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 507-16, (1997) (PubMed).

Détails sur CD59

Antigène: CD59

Autre désignation: CD59

Sujet: CD59 Molecule,CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.,MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

ID gène: 966

UniProt: P13987

Pathways: Système du Complément