FAS anticorps (FITC)

N° du produit ABIN94249

Cet anticorps Souris Monoclonal détecte spécifiquement FAS dans FACS. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour FAS anticorps (FITC) (ABIN94249)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: LT95

Détail du produit anti-FAS anticorps (FITC)

Fonction: Anti-Hu CD95 FITC

Specificité: The antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: HUT-78 human T cell lymphoma cell line

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-FAS (FITC) (ABIN94249)

Drosopoulos, Roberts, Cermak, Sasazuki, Shirasawa, Andera, Pintzas: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." dans: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, (2005) (PubMed).

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95

Sujet: Fas cell surface death receptor,CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.,FAS1, APT1, APO-1, FASTM, ALPS1A, TNFRSF6

ID gène: 355

UniProt: P25445

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity