RELB anticorps (AA 1-579)

N° du produit ABIN948396

Cet anticorps anti-RELB est un anticorps Souris Polyclonal détectant RELB dans WB et IF. Adapté pour Humain. Validé indépendamment pour une utilisation dans Immunofluorescence.

Aperçu rapide pour RELB anticorps (AA 1-579) (ABIN948396)

Antigène: RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))

Reactivité: Humain

Hôte: Souris

Clonalité: Polyclonal

Conjugué: Cet anticorp RELB est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-RELB anticorps

Épitope: AA 1-579

Fonction: Mouse polyclonal antibody raised against a full-length human RELB protein.

Séquence: MLRSGPASGP SVPTGRAMPS RRVARPPAAP ELGALGSPDL SSLSLAVSRS TDELEIIDEY IKENGFGLDG GQPGPGEGLP RLVSRGAASL STVTLGPVAP PATPPPWGCP LGRLVSPAPG PGPQPHLVIT EQPKQRGMRF RYECEGRSAG SILGESSTEA SKTLPAIELR DCGGLREVEV TACLVWKDWP HRVHPHSLVG KDCTDGICRV RLRPHVSPRH SFNNLGIQCV RKKEIEAAIE RKIQLGIDPY NAGSLKNHQE VDMNVVRICF QASYRDQQGQ MRRMDPVLSE PVYDKKSTNT SELRICRINK ESGPCTGGEE LYLLCDKVQK EDISVVFSRA SWEGRADFSQ ADVHRQIAIV FKTPPYEDLE IVEPVTVNVF LQRLTDGVCS EPLPFTYLPR DHDSYGVDKK RKRGMPDVLG ELNSSDPHGI ESKRRKKKPA ILDHFLPNHG SGPFLPPSAL LPDPDFFSGT VSLPGLEPPG GPDLLDDGFA YDPTAPTLFT MLDLLPPAPP HASAVVCSGG AGAVVGETPG PEPLTLDSYQ APGPGDGGTA SLVGSNMFPN HYREAAFGGG LLSPGPEAT

Réactivité croisée: Humain

Attributs du produit: Antibody reactive against mammalian transfected lysate.

Immunogène: RELB (NP_006500.2, 1 a.a. ~ 579 a.a) full-length human protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Independent Validation (IF)

Validation #104077 (Immunofluorescence)

by: Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum

No.: #104077

Date: 21.11.2019

Antigène: RELB

Numéro du lot: 10307

Application validée: Immunofluorescence

Contrôle positif:

U-937 human monocytic cell line

Contrôle négative:

No primary antibody control

Conclusion:

Passed. ABIN948396 specifically stains RelB in U-937 human monocytic cells.

Validation Images

U937 monocytic cells stained with with anti-RelB antibody ABIN948396 and an AF488-labeled secondary antibody (bottom row, green). Counterstain with DAPI (blue). Negative control without primary antibody (top row) shows no AF488 signal.

Protocole

Anticorps primaire: ABIN948396

Anticorps secondaire: AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214)

Full Protocol:

• Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10 % FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO₂ in 300µL in 24-well plates (Sarstedt AG&Co. KG).
• Use cytospin to spin cells onto slide.
• Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
• Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
• Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
• Wash cells 3x for 5min with PBS.
• Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
• Incubate cells with primary mouse anti-human RelB antibody (antibodies-online, ABIN948396, lot 10307) diluted 1:50 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
• Wash cells 3x for 5min with PBS.
• Incubate cells with secondary AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214) diluted 1:400 in 1x Duolink Antibody diluent for 1h at RT in the dark.
• Wash cells 2x for 5min with PBS.
• Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
• Image acquisition with an Olympus widefield microscope.

Notes:

Strong green staining dependent on the presence of ABIN948396 indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence.

Stockage

Buffer: In 1x PBS, pH 7.4

Conseil sur la manipulation: Aliquot to avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Détails sur RELB

Antigène: RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))

Autre désignation: RELB

Sujet: Full Gene Name: v-rel reticuloendotheliosis viral oncogene homolog B
Synonyms: I-REL,IREL

ID gène: 5971

NCBI Accession: NM_006509

Pathways: Signalisation NF-kappaB, Signalisation RTK