PUMA anticorps (AA 2-16)

N° du produit ABIN959855

L’anticorps Lapin Polyclonal anti-PUMA a été validé pour WB, IHC et IHC (p). Il convient pour détecter PUMA dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour PUMA anticorps (AA 2-16) (ABIN959855)

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUMA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PUMA anticorps

Épitope: AA 2-16

Marque: IHC-plus™

Specificité: Recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kD pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Is expected to recognize the canonical isofom 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%) Opossum (87%).

Purification: Purified

Immunogène: Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%), Opossum (87%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western Blot: detects a band of approximately 23kD in K562 cell lysates.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur PUMA

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Autre désignation: PUMA

Sujet: Name/Gene ID: BBC3
Family: Apoptosis

Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA

ID gène: 27113

UniProt: Q9BXH1

Pathways: Signalisation p53, Positive Regulation of Endopeptidase Activity