Caspase 2 anticorps (N-Term)

N° du produit ABIN96091

Détail du produit anti-Caspase 2 anticorps

Antigène: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Épitope: N-Term

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 2 est non-conjugé

Application: Western Blotting (WB), ELISA

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: A synthetic peptide corresponding to the of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.

Isotype: IgG

Information d'application

Indications d'application: This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2.  A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2.  No reaction was observed against human caspase-2.  Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2.This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product.  A 1:750 dilution is recommended for immunoblot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.44 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur Caspase 2

Antigène: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Autre désignation: Caspase-2 (CASP2 Produits)

Synonymes: anticorps xCaspase-2, anticorps Caspase-2, anticorps ICH-1, anticorps Nedd2, anticorps CASP-2, anticorps ICH1, anticorps NEDD-2, anticorps NEDD2, anticorps PPP1R57, anticorps ICH-1L/1S, anticorps ICH1L1S, anticorps caspase 2, anticorps caspase 2 L homeolog, anticorps caspase-2, anticorps CASP2, anticorps casp2.L, anticorps CpipJ_CPIJ008254, anticorps Casp2

Sujet: Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure.  Disruption of apoptosis may contribute to cancer and other diseases.  Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse.  Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis.  The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits.  The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.  Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B.  Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7.  Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue.  Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2.  These forms show tissue specificity and function either as inducers or suppressors of apoptosis.  Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.
Synonyms: Apoptosis related cysteine peptidase antibody, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Developmentally down regulated 2 antibody, ICH 1 antibody

ID gène: 64314

UniProt: P55215

Pathways: Apoptose, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway