GM-CSF anticorps

N° du produit ABIN964660

N° du produit (Fournisseur): 209-401-314

Cet anticorps Lapin Polyclonal détecte spécifiquement GM-CSF dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour GM-CSF anticorps (ABIN964660)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GM-CSF est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-GM-CSF anticorps

N° du produit (Fournisseur): 209-401-314

Fournisseur: Rockland

Fonction: GM-CSF Antibody

Réactivité croisée (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids.

Attributs du produit: Synonyms: rabbit anti-GM-CSF Antibody, GM-CSF, rabbit anti-Colony-stimulating factor, CSF, sargramostim and molgramostin

Purification: This purified antibody has been heated to 56°C for 30 minutes.

Immunogène:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This purified antibody has been tested for use in western blotting. Reactivity is also expected in neutralizations, ELISA, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 14.5 kDa in size corresponding to human GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GM-CSF

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: CSF2

Sujet: Background: Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.

ID gène: 1437

NCBI Accession: NP_000749

UniProt: P04141

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin