POLB anticorps (C-Term)

N° du produit ABIN964928

Détail du produit anti-POLB anticorps

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLB est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with overexpressed and endogenous POLß protein. A BLAST analysis was used to suggest reactivity with POLß from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLß from other sources has not been determined.

Attributs du produit: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptidecorresponding to a region near the C-terminus of the POLß protein.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: POLB

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.4 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-POLB (ABIN964928)

Asagoshi, Liu, Masaoka, Lan, Prasad, Horton, Brown, Wang, Bdour, Sobol, Taylor, Yasui, Wilson: "DNA polymerase beta-dependent long patch base excision repair in living cells." dans: DNA repair, Vol. 9, Issue 2, pp. 109-19, (2010) (PubMed).

Sobol, Foley, Nyska, Davidson, Wilson: "Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice." dans: DNA repair, Vol. 2, Issue 5, pp. 609-22, (2004) (PubMed).

Détails sur POLB

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Autre désignation: POLB (POLB Produits)

Synonymes: anticorps wu:fa97a05, anticorps zgc:109924, anticorps POLB, anticorps DDBDRAFT_0186152, anticorps DDBDRAFT_0215784, anticorps DDBDRAFT_0232376, anticorps DDB_0186152, anticorps DDB_0215784, anticorps DDB_0232376, anticorps A430088C08Rik, anticorps polymerase (DNA directed), beta, anticorps DNA polymerase beta, anticorps DNA polymerase X family protein, anticorps polymerase (DNA directed), beta S homeolog, anticorps polb, anticorps POLB, anticorps polB, anticorps polb.S, anticorps Polb

Sujet: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.
Synonyms: DNA polymerase beta, POLB, beta-pol

ID gène: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: Réparation de l'ADN