GDF15 anticorps (C-Term)

N° du produit ABIN964967

N° du produit (Fournisseur): 610-401-b87

Cet anticorps anti-GDF15 est un anticorps Lapin Polyclonal détectant GDF15 dans WB et ELISA. Adapté pour Humain et Souris.

Aperçu rapide pour GDF15 anticorps (C-Term) (ABIN964967)

Antigène: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GDF15 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-GDF15 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 610-401-b87

Fournisseur: Rockland

Fonction: GDF15 Antibody

Réactivité croisée (Details): This antibody reacts with endogenous NAG-1 protein from mouse and human tissues.

Attributs du produit: Synonyms: rabbit anti-Nag1 Antibody, NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a peptide corresponding to an amino acid sequence near the C-terminal of mouse NAG-1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified NAG1/GDF15 antibody has been tested by ELISA and western blotting of mouse and human NAG-1 protein.  For detection of NAG-1 in mouse serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal) specific antibodies.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2000 for human NAG-1, 1:3000-1:7000 for mouse NAG-1

ELISA Dilution: 1:100,000 - 1:120,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GDF15

Antigène: GDF15 (Growth Differentiation Factor 15 (GDF15))

Autre désignation: Gdf15

Sujet: Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

ID gène: 23886

NCBI Accession: NP_035949

UniProt: Q9Z0J7

Pathways: SARS-CoV-2 Protein Interactome