CD25 anticorps

N° du produit ABIN965780

L’anticorps Souris Monoclonal anti-CD25 a été validé pour FACS, IHC (fro) et IHC (af). Il convient pour détecter CD25 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour CD25 anticorps (ABIN965780)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Acetone-fixed) (IHC (af))

Clone: B489 (143-13)

Détail du produit anti-CD25 anticorps

Specificité: This antibody reacts a 55kD single chain glycoprotein, CD25, that associates with CD12 and CD132 to form the IL-2 receptor complex. CD25 has three epitope regions - A, B, and C - the latter of which is recognized by B489. This MAb has been clustered at the Fourth International Workshop on human differentiation antigens.

Purification: Concentrated.

Immunogène: Human leucocytes.

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (acetone fixed frozen tissue): 1:50-1:100
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen tissue sections (acetone fixed). The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Commentaires:

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-CD25 (ABIN965780)

Parke: "Intravenous gammaglobulin in pregnancy, the Connecticut experience." dans: Scandinavian journal of rheumatology. Supplement, Vol. 107, pp. 103-4, (1998) (PubMed).

Goldstein, Marcon, Cullen, Nelson: "A competitive enzyme-linked immunoassay (ELISA) for the measurement of soluble human interleukin-2 receptors (IL-2R, Tac protein)." dans: Journal of immunological methods, Vol. 107, Issue 1, pp. 103-9, (1988) (PubMed).

Uchiyama, Nelson, Fleisher, Waldmann: "A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 4, pp. 1398-403, (1981) (PubMed).

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25 (IL-2R)

ID gène: 3559

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation