EIF4E anticorps

N° du produit ABIN966052

Cet anticorps Souris Monoclonal détecte spécifiquement EIF4E dans WB, IHC, ELISA, IF et FACS. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour EIF4E anticorps (ABIN966052)

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EIF4E est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 5D11

Détail du produit anti-EIF4E anticorps

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of human EIF4E expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-EIF4E (ABIN966052)

Slepenkov, Korneeva, Rhoads: "Kinetic mechanism for assembly of the m7GpppG.eIF4E.eIF4G complex." dans: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25227-37, (2008) (PubMed).

Détails sur EIF4E

Antigène: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Autre désignation: EIF4E

Sujet: EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Synonyms: CBP, EIF4F, EIF4E1, EIF4EL1, MGC111573, EIF4E

Poids moléculaire: 25kDa

ID gène: 1977

Pathways: BCR Signaling