PUMA anticorps (C-Term)

N° du produit ABIN966924

Cité dans 1 publication. Cet anticorps anti-PUMA Polyclonal Lapin (ABIN966924) détecte spécifiquement PUMA dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour PUMA anticorps (C-Term) (ABIN966924)

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUMA est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PUMA anticorps

Épitope: C-Term

Réactivité croisée: Souris, Rat (Rattus)

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Bcl-2-binding component 3 (BBC3) detection. Tested with WB in Human, Mouse, Rat.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.

Isotype: IgG

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

Références pour anticorps anti-PUMA (ABIN966924)

Chipuk, Bouchier-Hayes, Kuwana, Newmeyer, Green: "PUMA couples the nuclear and cytoplasmic proapoptotic function of p53." dans: Science (New York, N.Y.), Vol. 309, Issue 5741, pp. 1732-5, (2005) (PubMed).

Détails sur PUMA

Antigène: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Autre désignation: Bcl-2-binding component 3 (BBC3)

Sujet: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Pathways: Signalisation p53, Positive Regulation of Endopeptidase Activity