BBC3 anticorps (BCL2 Binding Component 3) (C-Term)

Details for Product anti-BBC3 Antibody No. ABIN966924, Fournisseur: Connectez-vous pour afficher
Antigène
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Épitope
C-Term
104
79
17
16
13
12
9
9
8
8
7
5
3
2
2
2
1
1
1
1
1
1
1
1
Reactivité
Humain
331
142
130
36
1
1
1
Hôte
Lapin
297
24
16
Clonalité
Polyclonal
Conjugué
Cet anticorp BBC3 est non-conjugé
16
13
12
11
11
9
6
6
6
6
6
6
6
6
6
6
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
318
114
80
61
52
31
22
20
13
8
8
2
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.
Isotype IgG
 Réactivité croisée Souris, Rat (Rattus)
Réactivité croisée (Details) No cross reactivity with other proteins.
Attributs du produit Rabbit IgG polyclonal antibody for Bcl-2-binding component 3 (BBC3) detection. Tested with WB in Human, Mouse, Rat.
Plasmids, Primers & others Plasmids, Primers & others BBC3 products on genomics-online (e.g. as negative or positive controls)
Autre désignation Bcl-2-binding component 3 (BBC3) (BBC3 Antibody Extrait)
Sujet The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
Pathways Signalisation p53, Positive Regulation of Endopeptidase Activity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Agent conservateur Sodium azide, Thimerosal (Merthiolate)
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock -20 °C
Stockage commentaire At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Produit citée dans: Chipuk, Bouchier-Hayes, Kuwana, Newmeyer, Green: "PUMA couples the nuclear and cytoplasmic proapoptotic function of p53." dans: Science (New York, N.Y.), Vol. 309, Issue 5741, pp. 1732-5, 2005 (PubMed).

Background publications Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." dans: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Yu, Zhang, Hwang, Kinzler, Vogelstein: "PUMA induces the rapid apoptosis of colorectal cancer cells." dans: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Han, Flemington, Houghton, Gu, Zambetti, Lutz, Zhu, Chittenden: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

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