CD47 anticorps

N° du produit ABIN967480

Cet anticorps Rat Monoclonal détecte spécifiquement CD47 dans FACS, WB, IHC et FM. Il présente une réactivité envers Souris et a été mentionné dans 6+ publications.

Aperçu rapide pour CD47 anticorps (ABIN967480)

Antigène: CD47

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: Miap301

Détail du produit anti-CD47 anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Not reported

Isotype: IgG2a kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD47 (ABIN967480)

Gresham, Dale, Potter, Chang, Vines, Lowell, Lagenaur, Willman: "Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr." dans: The Journal of experimental medicine, Vol. 191, Issue 3, pp. 515-28, (2000) (PubMed).

Mi, Jiang, Weng, Lindberg, Narayanan, Lagenaur: "Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina." dans: The Journal of comparative neurology, Vol. 416, Issue 3, pp. 335-44, (2000) (PubMed).

Oldenborg, Zheleznyak, Fang, Lagenaur, Gresham, Lindberg: "Role of CD47 as a marker of self on red blood cells." dans: Science (New York, N.Y.), Vol. 288, Issue 5473, pp. 2051-4, (2000) (PubMed).

Jiang, Lagenaur, Narayanan: "Integrin-associated protein is a ligand for the P84 neural adhesion molecule." dans: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 559-62, (1999) (PubMed).

Lindberg, Bullard, Caver, Gresham, Beaudet, Brown: "Decreased resistance to bacterial infection and granulocyte defects in IAP-deficient mice." dans: Science (New York, N.Y.), Vol. 274, Issue 5288, pp. 795-8, (1996) (PubMed).

Reinhold, Lindberg, Plas, Reynolds, Peters, Brown: "In vivo expression of alternatively spliced forms of integrin-associated protein (CD47)." dans: Journal of cell science, Vol. 108 ( Pt 11), pp. 3419-25, (1996) (PubMed).

Détails sur CD47

Antigène: CD47

Autre désignation: CD47 (IAP)

Sujet: The miap301 antibody reacts with the extracellular domain of CD47, also known as Integrin-Associated Protein (IAP).1 CD47 is a 50-kDa plasma membrane protein with an extracellular immunoglobulin variable region-like domain, a multiple membrane-spanning segment, and a short C-terminal cytoplasmic tail. IAP is often physically associated with beta3 integrins, and it may act as a transducer element in activation mediated by these integrins.CD47 is expressed by a wide variety of cell types, including some cells lacking integrins, such as erythrocytes. The cytoplasmic tail region is expressed as alternatively spliced forms, which are differentially expressed in diverse tissues, suggesting that IAP may have distinct functions in various tissues. CD47 and its ligand, CD172a, are proposed to mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages.
Synonyms: IAP