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Nestin anticorps

NES Reactivité: Rat WB, IHC, FACS, FM Hôte: Souris Monoclonal Rat 401 unconjugated
N° du produit ABIN967492
  • Antigène Voir toutes Nestin (NES) Anticorps
    Nestin (NES)
    Reactivité
    • 132
    • 69
    • 50
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Hôte
    • 103
    • 38
    • 4
    • 2
    • 1
    Souris
    Clonalité
    • 108
    • 40
    Monoclonal
    Conjugué
    • 65
    • 10
    • 10
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Cet anticorp Nestin est non-conjugé
    Application
    • 81
    • 50
    • 48
    • 48
    • 41
    • 40
    • 39
    • 29
    • 25
    • 15
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    3. Please refer to us for technical protocols.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Rat (E15) spinal cord extracts
    Clone
    Rat 401
    Isotype
    IgG1
    Top Product
    Discover our top product NES Anticorps primaire
  • Commentaires

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Coppola, Dautréaux, Nomaksteinsky, Brunet: "Phox2b expression in the taste centers of fish." dans: The Journal of comparative neurology, Vol. 520, Issue 16, pp. 3633-49, (2013) (PubMed).

    Tseng, Gruzdeva, Li, Chuang, Sung: "Identification of the Tctex-1 regulatory element that directs expression to neural stem/progenitor cells in developing and adult brain." dans: The Journal of comparative neurology, Vol. 518, Issue 16, pp. 3327-42, (2010) (PubMed).

    Naritsuka, Sakai, Hashikawa, Mori, Yamaguchi: "Perisomatic-targeting granule cells in the mouse olfactory bulb." dans: The Journal of comparative neurology, Vol. 515, Issue 4, pp. 409-26, (2009) (PubMed).

    Tran, Banisadr, Ren, Chenn, Miller: "Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brain." dans: The Journal of comparative neurology, Vol. 500, Issue 6, pp. 1007-33, (2007) (PubMed).

    Pecchi, Dallaporta, Charrier, Pio, Jean, Moyse, Troadec: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." dans: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).

    Horky, Galimi, Gage, Horner: "Fate of endogenous stem/progenitor cells following spinal cord injury." dans: The Journal of comparative neurology, Vol. 498, Issue 4, pp. 525-38, (2006) (PubMed).

    Sadgrove, Laskowski, Gray et al.: "Examination of granule layer cell count, cell density, and single-pulse BrdU incorporation in rat organotypic hippocampal slice cultures with respect to culture medium, septotemporal position, and ..." dans: The Journal of comparative neurology, Vol. 497, Issue 3, pp. 397-415, (2006) (PubMed).

    Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." dans: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

    Tohyama, Lee, Rorke, Marvin, McKay, Trojanowski: "Monoclonal antibodies to a rat nestin fusion protein recognize a 220-kDa polypeptide in subsets of fetal and adult human central nervous system neurons and in primitive neuroectodermal tumor cells." dans: The American journal of pathology, Vol. 143, Issue 1, pp. 258-68, (1993) (PubMed).

    Dahlstrand, Collins, Lendahl: "Expression of the class VI intermediate filament nestin in human central nervous system tumors." dans: Cancer research, Vol. 52, Issue 19, pp. 5334-41, (1992) (PubMed).

    Tohyama, Lee, Rorke, Marvin, McKay, Trojanowski: "Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 66, Issue 3, pp. 303-13, (1992) (PubMed).

    Renfranz, Cunningham, McKay: "Region-specific differentiation of the hippocampal stem cell line HiB5 upon implantation into the developing mammalian brain." dans: Cell, Vol. 66, Issue 4, pp. 713-29, (1991) (PubMed).

    Lendahl, Zimmerman, McKay: "CNS stem cells express a new class of intermediate filament protein." dans: Cell, Vol. 60, Issue 4, pp. 585-95, (1990) (PubMed).

    Geschwind, Hockfield: "Identification of proteins that are developmentally regulated during early cerebral corticogenesis in the rat." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 9, Issue 12, pp. 4303-17, (1990) (PubMed).

    Frederiksen, McKay: "Proliferation and differentiation of rat neuroepithelial precursor cells in vivo." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 4, pp. 1144-51, (1988) (PubMed).

    Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

  • Antigène
    Nestin (NES)
    Autre désignation
    Nestin (NES Produits)
    Synonymes
    anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes
    Sujet
    Multipotential stem cells in the neural tube of the developing embryo give rise to the different neuronal cell types of the brain. Nestin is an intermediate filament protein which is abundantly expressed in neuroepithelial stem cells early in embryogenesis, but is absent from nearly all mature central nervous system (CNS) cells. After its down-regulation, GFAP and neurofilaments are expressed in differentiated astrocytes and neurons, respectively. Initial studies showed that the mouse anti-rat nestin antibody (clone Rat 401) recognized transient radial glial cells and dividing neuroepithelial stem cells in the embryonic rat CNS. Reportedly, Rat 401 has been used to analyze nestin expression in the developing rat nervous system and in immortalized CNS precursor cell lines (e.g such as in E11 rat CNS stem cells, but lost by postnatal day 6 (P6) in spinal cord and by P21 in the cerebellum). Although not expressed in normal adult CNS cells, nestin has been reportedly to be detectable in a variety of CNS tumors, suggesting that these tumors share gene expression patterns with primitive, undifferentiated CNS cells. Rat 401 positive cells have been reported to be found throughout the developing neural tube, but not in the adult CNS. More than 90% of dissociated E11 neural tube cells have been reported to be Rat 401 positive. Rat 401 recognizes rat nestin and has been reported not to cross-react with human nestin. Due to differential tissue expression, this antibody may recognize nestin as a doublet within a range of 198-260 kD.
    Poids moléculaire
    198-260 kDa
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