Caspase 4 anticorps

N° du produit ABIN967534

Cet anticorps Souris Monoclonal détecte spécifiquement Caspase 4 dans WB. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour Caspase 4 anticorps (ABIN967534)

Antigène: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 4 est non-conjugé

Application: Western Blotting (WB)

Clone: B25-1

Détail du produit anti-Caspase 4 anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant Human Caspase-4

Isotype: IgG1 kappa

Information d'application

Indications d'application: Applications include western blot analysis (1-2 µg/ml). Jurkat T cells (ATCC TIB- 152) and 293 adenovirus-transformed human kidney cells (ATCC CRL-1573) are suggested as positive controls.

Commentaires:

Related Products: ABIN967389, ABIN968537

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Caspase 4 (ABIN967534)

Faucheu, Diu, Chan, Blanchet, Miossec, Hervé, Collard-Dutilleul, Gu, Aldape, Lippke: "A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells." dans: The EMBO journal, Vol. 14, Issue 9, pp. 1914-22, (1995) (PubMed).

Munday, Vaillancourt, Ali, Casano, Miller, Molineaux, Yamin, Yu, Nicholson: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." dans: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, (1995) (PubMed).

Détails sur Caspase 4

Antigène: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Autre désignation: Caspase-4

Sujet: Caspase-4 (ICErelII, TX, ICH-2) is a 43 kDa cytosolic protein with homology to the ICE/Ced-3 family of cysteine proteases. Functional similarity with ICE proteases include the ability of Caspase-4 to induce apoptosis when overexpressed. Caspase-4 exhibits protease activity, cleaving both itself and the p30 ICE/caspase-1 proenzyme. However, unlike ICE/caspase-1, caspase-4 displays no IL-1beta processing activity. Caspase-4 mRNA is expressed in most adult human tissues, suggesting a broad tissue distribution. The B25-1 antibody recognizes an ~43 kDa band corresponding to human Caspase-4 (TX). A recombinant human Caspase-4 protein fragment corresponding to amino acids 334-377 was used as immunogen.
Synonyms: TX, Ich-2

Poids moléculaire: 43 kDa

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity