Cyclin D1 anticorps (full length)

N° du produit ABIN967539

Cet anticorps anti-Cyclin D1 est un anticorps Souris Monoclonal détectant Cyclin D1 dans WB, IP, IHC (f), IHC (fro) et ICS. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour Cyclin D1 anticorps (full length) (ABIN967539)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS)

Clone: DCS-6

Détail du produit anti-Cyclin D1 anticorps

Épitope: full length

Marque: BD Pharmingen™

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Full-length Cyclin D1 Recombinant Protein

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968585, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Cyclin D1 (ABIN967539)

Darzynkiewicz, Gong, Juan, Ardelt, Traganos: "Cytometry of cyclin proteins." dans: Cytometry, Vol. 25, Issue 1, pp. 1-13, (1997) (PubMed).

de Boer, Schuuring, Dreef, Peters, Bartek, Kluin, van Krieken: "Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma." dans: Blood, Vol. 86, Issue 7, pp. 2715-23, (1995) (PubMed).

Lukas, Pagano, Staskova, Draetta, Bartek: "Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines." dans: Oncogene, Vol. 9, Issue 3, pp. 707-18, (1994) (PubMed).

Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." dans: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, (1994) (PubMed).

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: Cyclin D1

Sujet: Cyclins and cyclin-dependent kinases (Cdks) have been shown to be subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Specific substrates for Cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are involved in regulating the passage of mammalian cells through G1. In SDS-PAGE, D-type cyclins migrate at the following molecular weights: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 [31 and 34 kDa (doublet)]. Rodent cyclin D1 homologues (Cyl1) have been reported to typically migrate as a 36 kDa doublet. The mouse anti-human cyclin D1 antibody (clone DCS-6) recognizes human cyclin D1 (36 kDa) and has been reported to crossreact with rat and mouse cyclin D1 homologues (Cyl1). It does not crossreact with human cyclins D2 and D3.

Poids moléculaire: 36 kDa

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling