Cortactin anticorps

N° du produit ABIN967709

L’anticorps Souris Monoclonal anti-Cortactin a été validé pour WB, IP, IF et IHC (f). Il convient pour détecter Cortactin dans des échantillons de Humain et Chien. Il y a 5+ publications disponibles.

Aperçu rapide pour Cortactin anticorps (ABIN967709)

Antigène: Cortactin (CTTN)

Reactivité: Humain, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cortactin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 30-Cortactin

Détail du produit anti-Cortactin anticorps

Réactivité croisée: Humain, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Chicken Cortactin

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Cortactin (ABIN967709)

Lavoie, Champagne, Gingras, Robert: "Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases." dans: The Journal of cell biology, Vol. 150, Issue 5, pp. 1037-56, (2000) (PubMed).

Nakahara, Mueller, Nomizu, Yamada, Yeh, Chen: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." dans: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, (1998) (PubMed).

Dehio, Prévost, Sansonetti: "Invasion of epithelial cells by Shigella flexneri induces tyrosine phosphorylation of cortactin by a pp60c-src-mediated signalling pathway." dans: The EMBO journal, Vol. 14, Issue 11, pp. 2471-82, (1995) (PubMed).

Wu, Parsons: "Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex." dans: The Journal of cell biology, Vol. 120, Issue 6, pp. 1417-26, (1993) (PubMed).

Wu, Reynolds, Kanner, Vines, Parsons: "Identification and characterization of a novel cytoskeleton-associated pp60src substrate." dans: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5113-24, (1991) (PubMed).

Détails sur Cortactin

Antigène: Cortactin (CTTN)

Autre désignation: Cortactin

Sujet: Cortactin is a protein which is represented by two structurally related isoforms of 80 kDa and 85 kDa. Sequence analysis of this protein reveals the presence of a carboxy-terminal SH3 domain, as well as six internal tandem repeats of a 37 amino acid sequence. It is able to bind to F-actin via this N-terminal repeat region, and colocalizes with actin in the cell. Cortactin was originally described as a protein that is tyrosine-phosphorylated in cells transformed by the src oncogene, in which it localizes to rosettes or podosomes. It has since been shown that other stimuli, such as bacterial invasion, treatment with growth factors, and integrin-mediated cell adhesion, are also capable of inducing phosphorylation of cortactin. Some additional evidence indicates that cortactin may actually be a substrate of syk, with src acting as an upstream signaling molecule. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 85/80 kDa

Pathways: Signalisation MAPK